Open AccessGENETIC ANALYSIS ON HIBISCUS SPECIES BY USING RAPD MARKERS
Author(s) -
Shilpa Kadve,
Mukesh Yadav,
Archana Tiwari
Publication year - 2012
Publication title -
international journal of biomedical and advance research
Language(s) - English
Resource type - Journals
eISSN - 2455-0558
pISSN - 2229-3809
DOI - 10.7439/ijbar.v3i6.472
Subject(s) - rapd , hibiscus , biology , genetic analysis , botany , genetic marker , dna extraction , traditional medicine , genetics , genetic diversity , gene , polymerase chain reaction , medicine , population , environmental health
This studies was undertaken for genetic analysis within the 7 varieties of Hibiscus rosa-sinensis by using RAPD markers the method involves a extraction of DNA from leaves of Hibiscus rosa-sinensis and yield of DNA ranged from 158-200µg and purity (ratio) was between 1.3-1.6 indicating minimum level of contaminating metabolites. Genetic analysis and relationship among seven Hibiscus germplasm were analyzed using Random Amplified Polymorphic DNA (RAPD). Total nine primers were used as, RPI-07, RPI-08, RPI-10, RPI-12, RPI-18, RPI-19, RPI-21, RPI-22 and RPI-23. No PCR product was generated through the primer RPI-23 indicating that no binding sites for this primer. In all the primers RPI-07, RPI-08, RPI-10, RPI-19 and RPI-21 are more suitable for the genetic variation analysis in Hibiscus species as it has shown maximum polymorphic bands. Primers RPI-12, RPI-18 and RPI-22 are weak in showing the polymorphic banding pattern for the all species of Hibiscus rosa-sinensis