Open AccessWound infection (with both Gram-positive and Gram-negative organisms) is a major problem in delayed wound healing. Persistence of these organisms in the wound delays considerably the healing time and increases length of hospital stay. Ultraviolet-C has shown to effectively eliminate these organisms (bactericidal effect) in few laboratory, animal and human studies and has shown promising role in promoting wound healing. Several wound dressing methods have been used to accelerate the rate of wound healing. Among them, Limited Access Dressing (LAD) utilizes intermittent negative pressure dressing using a transparent plastic cover combined with moist wound healing principle, minimizes need for regular change of dressing. Thus, the present study was conducted to explore the in-vitro bactericidal effect of direct and filtered UVC through transparent plastic sheet on Gram-negative bacilli.