DYNAMIC IMAGING OF INTRACELLULAR CA2+ CONCENTRATION IN INSULIN SECRETING MIN6 Î’-CELLS USING WIDE FIELD AND TIRF MICROSCOPY | Zendy

Shiv Kumar Mishra | Zendy; Priyanka Sharma | Zendy; Vinita Pawar | Zendy; Archana Tiwari | Zendy

Open Access

DYNAMIC IMAGING OF INTRACELLULAR CA2+ CONCENTRATION IN INSULIN SECRETING MIN6 Î’-CELLS USING WIDE FIELD AND TIRF MICROSCOPY

Author(s) -

Shiv Kumar Mishra,

Priyanka Sharma,

Vinita Pawar,

Archana Tiwari

Publication year - 2012

Publication title -

international journal of biomedical and advance research

Language(s) - English

Resource type - Journals

eISSN - 2455-0558

pISSN - 2229-3809

DOI - 10.7439/ijbar.v3i10.327

Subject(s) - intracellular , total internal reflection fluorescence microscope , microscopy , biophysics , insulin , chemistry , medicine , materials science , microbiology and biotechnology , pathology , biology

Since 1920's major medical advance based on animal research i.e. Insulin for diabetes. Diabetes is worldâ€™s most common metabolic disorder characterised by pancreatic Î²-cells dysfunction. Normally these cells respond to high glucose concentrations following exocytosis of stored insulin trigger by the combined action of store-operated calcium entry (SOCE) and calcium-release activated current (CRAC) channel, with an elusive mechanism. To illustrate it, dynamic imaging may used as power full tool as, it offers power of capturing the dynamics of biological action in live cells. In this review we focuses on dynamic imaging of pancreatic MIN6 cells, an appropriateÂ model used as an alternative to animal experiments in diabetes research to understand what is occurring, in and among pancreatic Î²-cells and influence their behaviour in desired ways, using advanced wide field and total internal reflection fluorescence microscopy (TIRFM). This may empower the principles of optimization of diabetes research.

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