Open AccessBiosynthesis and characterization of silver nanoparticles (AgNPs) using marine bacteria against certain human pathogens
Author(s) -
J. Nasrin Begam
Publication year - 2016
Publication title -
international journal of advances in scientific research
Language(s) - English
Resource type - Journals
ISSN - 2395-3616
DOI - 10.7439/ijasr.v2i7.3514
Subject(s) - silver nanoparticle , fourier transform infrared spectroscopy , nuclear chemistry , nanoparticle , scanning electron microscope , antibacterial activity , spectroscopy , chemistry , absorption spectroscopy , materials science , absorption (acoustics) , infrared spectroscopy , bacteria , nanotechnology , chemical engineering , organic chemistry , biology , physics , quantum mechanics , engineering , composite material , genetics
The present work investigates the synthesis of silver nanoparticles (AgNPs) by biological method using marine bacteria. The minimum inhibitory concentration (MIC) test was performed to find the inhibitory concentration of AgNO3 against marine bacterial isolate. After MIC study, the biogenic AgNPs was prepared through marine bacterial isolate and characterized by using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), X Ray Diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). From the UV-visible spectroscopy, the absorption peak was found at 420 nm. In SEM image, it is confirmed that the sample contains spherical shaped silver nanoparticles and most of the particles were below 100 nm in size. In XRD analysis, it was confirmed that the silver nanoparticles are crystalline in nature, which was confirmed by the FTIR peak at 518 cm-1 corresponding to the Ag vibration present in crystalline structure. The antimicrobial activity of silver nanoparticles was determined by disk diffusion method, and found that silver nanoparticles have significant antibacterial activity against most of the pathogens.