Open AccessEstimation of Levocetirizine in Bulk and Formulation by First Order Derivative Area under Curve UV-Spectrophotometric Methods.
Author(s) -
P Mane
Publication year - 2016
Publication title -
international journal of advances in scientific research
Language(s) - English
Resource type - Journals
ISSN - 2395-3616
DOI - 10.7439/ijasr.v2i1.2888
Subject(s) - levocetirizine , derivative (finance) , calibration curve , dosage form , second derivative , calibration , mathematics , area under curve , chromatography , pharmaceutical formulation , chemistry , analytical chemistry (journal) , statistics , detection limit , medicine , mathematical analysis , pharmacology , organic chemistry , financial economics , economics , pharmacokinetics
Simple, fast and reliable spectrophotometric methods were developed for determination of Levocetirizine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 235-243 nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Levocetirizine using 5-25?g/ml (r=0.9994) for first order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Levocetirizine in pharmaceutical formulations.