Open AccessZero Order and Area Under Curve Spectrophotometric methods for determination of Omeprazole Capsules in Pharmaceutical Formulation
Author(s) -
Santosh A. Jadhav,
Rekha Kharat,
Farhat Mujawar Pirjade,
Dilshadbe Tamboli
Publication year - 2015
Publication title -
international journal of advances in scientific research
Language(s) - English
Resource type - Journals
ISSN - 2395-3616
DOI - 10.7439/ijasr.v1i2.1854
Subject(s) - omeprazole , calibration curve , zero order , chromatography , dosage form , pharmaceutical formulation , standard curve , calibration , relative standard deviation , chemistry , mathematics , analytical chemistry (journal) , detection limit , first order , statistics , medicine , pharmacology
Simple, fast and reliable spectrophotometric methods were developed for determination of Omeprazole in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the zero order derivative values measured at 238 nm and the area under the curve method values measured at 300-305 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Omeprazole using 2-10?g/.ml (r=0.9985 and r=0.9959) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Omeprazole in tablets.