Open AccessDevelopment of Acrylic Acid Grafted Polycaprolactone (PCL)/Biphasic Calcium Phosphate (BCP) Nanofibers for Bone Tissue Engineering Using Gamma-Irradiation
Author(s) -
정진오,
정성린,
신영민,
박종석,
권희정,
안성준,
허중보,
신흥수,
임윤묵
Publication year - 2015
Publication title -
pollimeo/porrime
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.152
H-Index - 18
eISSN - 2234-8077
pISSN - 0379-153X
DOI - 10.7317/pk.2015.39.3.418
Subject(s) - polycaprolactone , nanofiber , electrospinning , biocompatibility , materials science , nuclear chemistry , acrylic acid , phosphate , bone tissue , chemical engineering , biomedical engineering , polymer chemistry , chemistry , composite material , organic chemistry , monomer , polymer , medicine , metallurgy , engineering
Polycaprolactone (PCL)? biphasic calcium phosphate (BCP)? ? ? ?? ?? ??? ?? ???? ??? ???? ??. ???,PCL? ??? ?? ??? ?? ? ??? ??? ?? ???? ?? ? ???? ???? ? ??? ?? ???? ??? PLC/BCP . ?? , ???? ???? acrylic acid (AAc)? . SEM? ?? ?? ?? ??? , AAc? ??? ??? MG63? ?? ???? ??? ??? ?? . ???? ??? 1.239 +/- 0.226 nmole/mu g/min?? ???? ?? ????(0.590 +/- 0.286 nmole/mu g/min)?? .??? AAc? ??? PCL/BCP ??? ??? ??? ? ?? ??? ????. Polycaprolactone (PCL) and biphasic calcium phosphate (BCP) have been considered as useful materials for orthopedic devices and osseous implants because of their biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces reducing initial cell adhesion or proliferation. To overcome the limitation, we fabricated surface-modified PCL/BCP nanofibers using gamma-irradiation for bone tissue engineering. PCL/BCP nanofibers were prepared by electrospinning and then we supplemented hydrophilicity by introducing acrylic acid (AAc) through gamma-irradiation. We confirmed the surface of nanofibers by SEM, and then the initial viability of MG63 was significantly increased on the AAc grafted nanofibers, and alkaline phosphatase activity(1.239 +/- 0.226 nmole/mu g/min) improved on the modified nanofibers than that on the non-modified nanofibers(0.590 +/- 0.286 nmole/mu g/min). Therefore, AAc-grafted nanofibers may be a good tool for bone tissue engineering applications.? ??? ???? (2012M2A2A6013196)? (HI14C0744)? ??? ?? ???? ???, ??