Open AccessNetwork Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells
Author(s) -
Zhifeng Du,
Bingli Wu,
JianJun Xie,
Xuan-Hao Lin,
XiaoYang Qiu,
Xia Zhan,
Shaohong Wang,
Jian Shen,
EnMin Li,
Li–Yan Xu
Publication year - 2015
Publication title -
asian pacific journal of cancer prevention
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 75
eISSN - 2476-762X
pISSN - 1513-7368
DOI - 10.7314/apjcp.2015.16.13.5445
Subject(s) - fascin , gene knockdown , biology , motility , microbiology and biotechnology , cell , cytoplasm , subcellular localization , actin cytoskeleton , cytoskeleton , actin , gene expression , gene , computational biology , genetics
Fascin-1 (FSCN1) is an actin-bundling protein that induces cell membrane protrusions, increases cell motility, and is overexpressed in various human epithelial cancers, including esophageal squamous cell carcinoma (ESCC). We analyzed various protein-protein interactions (PPI) of differentially-expressed genes (DEGs), in fascin knockdown ESCC cells, to explore the role of fascin overexpression. The node-degree distributions indicated these PPI sub-networks to be characterized as scale-free. Subcellular localization analysis revealed DEGs to interact with other proteins directly or indirectly, distributed in multiple layers of extracellular membrane-cytoskeleton/ cytoplasm-nucleus. The functional annotation map revealed hundreds of significant gene ontology (GO) terms, especially those associated with cytoskeleton organization of FSCN1. The Random Walk with Restart algorithm was applied to identify the prioritizations of these DEGs when considering their relationship with FSCN1. These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile following fascin knockdown to future examine the roles and mechanisms of fascin action.