Open AccessMiR-150-5p Suppresses Colorectal Cancer Cell Migration and Invasion through Targeting MUC4
Author(s) -
Weihua Wang,
Jie Chen,
Feng Zhao,
Bu-Rong Zhang,
Hongsheng Yu,
Hongfang Jin,
Jinyue Dai
Publication year - 2014
Publication title -
asian pacific journal of cancer prevention
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 75
eISSN - 2476-762X
pISSN - 1513-7368
DOI - 10.7314/apjcp.2014.15.15.6269
Subject(s) - metastasis , colorectal cancer , cell migration , cancer research , blot , biology , microrna , reporter gene , cell , untranslated region , cancer , gene , messenger rna , gene expression , genetics
Growing evidence suggests that miR-150-5p has an important role in regulating genesis of various types of cancer. However, the roles and the underlying mechanisms of miR-150-5p in development of colorectal cancer (CRC) remain largely unknown. Transwell chambers were used to analyze effects on cell migration and invasion by miR-150-5p. Quantitative real-time PCR (qRT-PCR), Western blotting and dual-luciferase 3' UTR reporter assay were carried out to identify the target genes of miR-150-5p. In our research, miR-150-5p suppressed CRC cell migration and invasion, and MUC4 was identified as a direct target gene. Its effects were partly blocked by re-expression of MUC4. In conclusiomn, miR-150-5p may suppress CRC metastasis through directly targeting MUC4, highlighting its potential as a novel agent for the treatment of CRC metastasis.