Open AccessQuantitative Real-Time RT-PCR of ITGA7, SVEP1, TNS1, LPHN3, SEMA3G, KLB and MMP13 mRNA Expression in Breast Cancer
Author(s) -
Manas Kotepui,
Charin Thawornkuno,
Porntip Chavalitshewinkoon-Petmitr,
Phaibul Punyarit,
Songsak Petmitr
Publication year - 2012
Publication title -
asian pacific journal of cancer prevention
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 75
eISSN - 2476-762X
pISSN - 1513-7368
DOI - 10.7314/apjcp.2012.13.11.5879
Subject(s) - breast cancer , real time polymerase chain reaction , messenger rna , reverse transcription polymerase chain reaction , gene expression , oncology , reverse transcriptase , gene , medicine , polymerase chain reaction , cancer , cancer research , biology , genetics
Breast cancer is the leading cause of cancer deaths among women worldwide, including Thailand. In the present study, the differential mRNA expression of SVEP1, LPHN3, KLB, ITGA7, SEMA3G, TNS1 and MMP13 genes was examined in breast cancer using quantitative real-time reverse transcription polymerase chain reaction (QRT-PCR). Among these genes, increased LPHN3 and MMP13 mRNA expression levels correlated with axillary-node metastasis (P=0.02). Multiple logistic regression analysis revealed that LPHN3 and MMP13 mRNA expression is significantly associated with axillary node status in breast cancer (P=0.04).