Open AccessALKALINE PHOSPHATASE ENZYME AND LACTATE DEHYDROGENASE ACTIVITY IN URINE OF PATIENTS TREATED WITH METHOTREXATE
Author(s) -
Tatjana Vujić,
Biljana Davidović Plavšić,
Snježana Uletilović,
Svjetlana Stoisavljević Šatara,
Jelica Predojević Samardžić,
Živko Saničanin
Publication year - 2014
Publication title -
contemporary materials
Language(s) - English
Resource type - Journals
eISSN - 1986-8677
pISSN - 1986-8669
DOI - 10.7251/comen1401146v
Subject(s) - methotrexate , lactate dehydrogenase , alkaline phosphatase , nephrotoxicity , creatinine , urine , enzyme , chemistry , medicine , endocrinology , biochemistry , kidney
In order study methotrexate nephrotoxicity, the activities of proximal tubule epithelial cell membrane enzymes: alkaline phosphatase (AP) and lactate dehydrogenase (LDH) were determined in 12-h-urine samples of 30 patients with lymphoblastomous leukemia. The patients were i.v. receiving 4 individual methotrexate doses of 2000 mg/m2 every 15 days followed by leucovorin as a protector. Control and methotrexate-treated group, each consisting of 30 examinees, included 4-10 years old children of both sexes. Statistically significant increase of AP and LDH activities, expressed as unuts/mmol creatinine was observed after the second therapy (p0.05) in relation to the control. Based on these results it can concluded that nephrotoxic methotrexate action is ireversible during the time period after the second applications at the level of proximal tubule epithelal cell.