Microbial Community Behaviour in The Rhizosphere of Kilemo (Litsea cubeba L. Pers) After Pruning

Pruning is a removing some parts of the tree canopy, either for improving tree shape or increasing shoot productivity.\udIt can be implemented in yield management of commodities that are harvested its shoot biomass, such as tea or\udkilemo. This activity interferes plant growth, hence affect root secretion. Consequently, it alters soil microbe\udpopulations in the rhizosphere. This paper observes the impacts of pruning on fluctuation of root exudates (soil\udsugar) and rhizosphere communities of 2-years-old kilemo (Litsea cubeba L. Pers.). Tree samples were pruned by\udremoving shoots above the lowest branches. A month after cutting, trees fertilized using 3 types of fertilizers: leaf,\udNPK, and organic fertilizer. As a control, there were non-fertilized treated trees. To observe the root exudation and\udth th th th\udmicrobes populations, 0−20 cm depth of soil at rhizospheres were collected from 10 , 30 , 60 , and 90 day after\udpruning. Samples were further analyzed for observing the amount of soil-sugar and microbes population, such as Nfixer, cellulose degrader, and phosphate solubilizer. Results show that kilemo root drastically decreased their sugar\udexudation by 22% (10th day), 28% (30th day), 44% (60th day), and 70% (90th day), respectively. Therefore, those\udpopulations were significantly depleted. Rhizosphere sugar can be improved by fertilization, however, the\udaugmentation did not enhance the rhizosphere communitie