Open AccessEffect of progesterone on the MGMT gene expression in MCF7, HEp-2 and 293 cells
Author(s) -
Z. M. Nidoieva,
A. P. Latsyshyna
Publication year - 2021
Publication title -
vìsnik ukraïnsʹkogo tovaristva genetikìv ì selekcìonerìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3680
pISSN - 1810-7834
DOI - 10.7124/visnyk.utgis.18.1-2.1350
Subject(s) - progesterone receptor , nuclear receptor , gene expression , downregulation and upregulation , western blot , messenger rna , receptor , biology , microbiology and biotechnology , steroid hormone , gene , cancer research , cancer , biochemistry , genetics , transcription factor , estrogen receptor , breast cancer
Aims: to investigate the steroid hormone progesterone effect on the human MGMT gene expression at the mRNA and protein levels in cell lines with different expression patterns of the nuclear progesterone receptors and membrane receptor PGRMC1. Methods: cell culture, RNA / protein isolation, cDNA synthesis, real-time polymerase chain reaction, Western blot analysis. Results: We observe the MGMT gene upregulation by progesterone at both mRNA and protein levels. Conclusions the effect of progesterone on MGMT expression is more complex than direct regulation through the classical nuclear receptor.Keywords: O6-methylguanine-DNA methyltransferase (MGMT), progesterone, nuclear progesterone receptors (nPR), progesterone receptor membrane component 1 (PGRMC1), gene expression regulation.