Open AccessOrganization of 5S ribosomal DNA of Litchi chinensis Sonn.
Author(s) -
O. O. Ishchenko,
V. V. Kozub,
І. І. Панчук
Publication year - 2021
Publication title -
vìsnik ukraïnsʹkogo tovaristva genetikìv ì selekcìonerìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3680
pISSN - 1810-7834
DOI - 10.7124/visnyk.utgis.18.1-2.1348
Subject(s) - biology , ribosomal dna , genetics , genome , ribosomal rna , 5s ribosomal rna , repeated sequence , dna , cloning (programming) , gene , phylogenetics , 18s ribosomal rna , computer science , programming language
Aim. 5S ribosomal DNA (5S rDNA) represents a universal model for studying the evolution of repeated sequences in eukaryotic organisms. Taking into account that this region of the genome still remains almost undescribed in species of the family Sapindaceae, we investigated the molecular organization of a repeated unit of 5S rDNA in a member of this family, Litchi chinensis. Methods. PCR amplification, cloning and sequencing of 5S rDNA. Results. It was found that the length of the repeated unit of the 5S rDNA of L. chinensis is 321 -323 bp. The level of intragenomic similarity of 5S rDNA repeats is 87.1 %. Potential external elements of the RNA polymerase III promoter, which are localized in IGS, differ from those described for members of other families of angiosperms. Conclusions. In the genome of L. chinensis, at least two classes of 5S rDNA repeats are present, which differ in the sequence of external promoter elements.
Keywords: 5S rDNA, molecular evolution, Litchi chinensis, Sapindaceae.