Open AccessThe interaction of verprolin WIRE with the adapter proteins family intersectin
Author(s) -
S. V. Kropyvko,
A. V. Rynditch
Publication year - 2019
Publication title -
vìsnik ukraïnsʹkogo tovaristva genetikìv ì selekcìonerìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3680
pISSN - 1810-7834
DOI - 10.7124/visnyk.utgis.17.1.1195
Subject(s) - signal transducing adaptor protein , cytoskeleton , actin cytoskeleton , actin , microbiology and biotechnology , adapter (computing) , endogeny , protein family , chemistry , biology , signal transduction , cell , genetics , biochemistry , gene , computer science , operating system
Aim. WIRE is a scafold protein that regulates actin cytoskeleton rearrangements and the formation of actin enriched membrane processes responsible for invasion and migration. ITSN1 and ITSN2 are representatives of the family of intersectins who participate in the reorganization of the actin cytoskeleton, as well as in other processes, such as endo/enzocytosis, cellular signal transduction, etc. As these proteins participate in the same processes, we checked their interaction with each other. Methods. Protein-protein interactions were identified using the GST pull-down method. Results. We showed that the SH3 domains of ITSN1 and ITSN2 interact with WIRE, and found that while WIRE is in a complex with endogenous actin. Conclusions. ITSN1 and ITSN2 interact with WIRE, which is located in a complex with endogenous actin. Keywords: WIRE, ITSN1 and ITSN2, actin.