Open AccessGenetic transformation of Populus nigra X P. deltoides (black poplar, clone Gradizka)
Author(s) -
N. K. Kutsokon,
V. A. Rudas,
M. V. Shinkaruk,
Olha Lakhneko,
B.V. Morgun,
Namik Rashydov,
D. M. Grodzynsky
Publication year - 2016
Publication title -
vìsnik ukraïnsʹkogo tovaristva genetikìv ì selekcìonerìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3680
pISSN - 1810-7834
DOI - 10.7124/visnyk.utgis.14.2.686
Subject(s) - kanamycin , transformation (genetics) , biology , clone (java method) , gene , petiole (insect anatomy) , botany , polymerase chain reaction , marker gene , genetic analysis , genetics , hymenoptera
Aim. To carry out genetic transformation of poplar Populus nigra x P. deltoides clone Gradizka with the model gene construct pCB002 carrying selective gene of kanamycin resistance and marker gene of β-glucuronidase. Methods. Genetic transformation was performed with the using leaf, stem and petiole poplar explants. Transformants were selected on the medium with kanamycin, and transgene was identified by polymerase chain reaction (PCR) and histochemical GUS assay. Results. Successful transformants selected on kanamycin media were confirmed by the presence of PCR-product for the gene nptII with the length 700 bp, and gus gene expression was also observed. Conclusions. Protocol for genetic transformation of P. nigra x P. deltoides clone Gradizka established here will be used for poplar genetic modification to create new clones with commercially important traits.Keywords: genetic transformation, Populus sp., microclonal propagation.