Open AccessIdentification of dehydrin genes Dhn1 and Zmdhn13 alleles in maize varieties and lines
Author(s) -
O. O. Pogrebniuk,
В. І. Файт
Publication year - 2021
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v28.1373
Subject(s) - biology , allele , locus (genetics) , gene , genetics , genotype , zea mays , polymerase chain reaction , agronomy
Aim. Determination of molecular genetic polymorphism of maize varieties and lines (Zea mays L.) of Ukrainian and world selection by Dhn1 and ZmDHN13 dehydrin genes. Methods. Polymerase chain reaction (PCR), PAAgel-electrophoresis. Results. Maize varieties and lines of different geographical origin and different years of creation were analyzed by Dhn1 and ZmDhn13 dehydrin genes. Conclusions. The use of directional primers revealed five different alleles of the Dhn1 locus, which differed in the size of the amplification product: 186, 190, 194, 196 and 200 bp. The most common allele was 196 bp (46.2%). Two alleles of the ZmDhn13 locus were detected: 86 bp and 82 bp. Allele frequency 86 bp was 61.5% and the allele frequency 82 bp was 38.5%. The sequences of the dehydrin genes Dhn1 and ZmDhn13 are not conservative, deletions and / or insertions within these genes are observed. Accordingly, the structure and functional activity of the dehydrin proteins that encode these genes in different genotypes can vary greatly.
Keywords: Zea mays L., dehydrins, genes, drought resistance.