Open AccessEvaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols
Author(s) -
О. V. Shcherbak,
С. И. Ковтун,
Олена Іванівна Метлицька,
P. A. Trotskyi,
Iryna Lyuta,
Oksana Lyzohub
Publication year - 2020
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v27.1341
Subject(s) - boar , cryopreservation , sperm , semen , andrology , biology , semen cryopreservation , breed , sperm quality , sperm motility , zoology , embryo , medicine , genetics
Aim. To evaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols to optimize biotechnological approaches in pig reproduction based on the genetic material of the Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of National Academy of Agrarian Science of Ukraine. Methods. Cryopreserved ejaculated boars' sperm was thawed in three different ways. Biotechnological, cryobiological and morphological methods were used to assess the viability of the sperm. Results. We noted the individual feature of semen quality of the studied boars, which affects its suitability for cryopreservation and quality indicators after thawing. It was found that in liquefied boar sperm (sperm activity on average is 86.7%) after its centrifugation and three-hour equilibration at + 4 °C sperm activity decreased by an average of 25.0%. In order to improve the quality of cryopreserved sperm, it was thawed under different conditions. The highest activity of spermatozoa in thawed sperm of boar No. 225 of Myrhorod pig was at the level of 25.0% at the thawing temperature + 70 °C, and the thermal resistance and heat resistance were 70.0% and 60.0%, respectively. Conclusions. During this study was noted that there are peculiarities of boar semen at breed and individual level, which influence its ability to tolerate cryopreservation. Keywords: sperm, in vitro cultivation, cryobank, cryopreservation, boars.