Open AccessGenetic diversity of spring common barley varieties at hordein loci
Author(s) -
L. V. Sirant,
N. V. Sandetska
Publication year - 2020
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v27.1336
Subject(s) - hordein , hordeum vulgare , allele , biology , genotype , genetic diversity , storage protein , hordeum , spring (device) , agronomy , genetics , poaceae , gene , population , mechanical engineering , demography , sociology , engineering
Aim. The aim of the study was to investigate the varieties of hordein loci of spring barley suitable for cultivation in Ukraine. Methods. To identify the genotype by the loci of the hordein, we used the method of separating the hordeins in polyacrylamide gel (PAAG) in acidic medium by the Brzezinski method with modifications. Results. In 26 varieties of spring barley, genotypes were identified by the hordein loci of Hrd A, Hrd B and HrdF. Differences between alleles of storage protein loci were found in the frequency with which they were distributed among varieties. Conclusions. Found a high level of allelic variability. In barley varieties there is a tendency for the distribution of certain alleles of loci of hordein storage proteins of barley: Hrd A (A2,A4, A12,A18, A23, A32), Hrd B (B1, B5, B6, B8, B17, B19, B21,B29, B35,B67, B164, ), Hrd F (F1, F2, F3, F0).
Keywords: Hordeum vulgare L., spring barley, storage proteins, hordeins, alleles.