Open AccessIdentification of leaf rust resistance gene Lr34/Yr18/Sr57/Pm38/Bdv1 in soft wheat varieties
Author(s) -
N. V. Sandetska,
O. M. Radchenko
Publication year - 2020
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v27.1317
Subject(s) - powdery mildew , biology , rust (programming language) , microsatellite , locus (genetics) , cultivar , allele , horticulture , polymerase chain reaction , gene , stripe rust , plant disease resistance , genetic marker , agronomy , genetics , computer science , programming language
Aim. Analysis of the allelic composition of the csLV34 locus and the microsatellite locus Xgwm295 for the detection of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene, which determines the resistance of modern wheat varieties of domestic breeding of diseases: leaf rust and powdery mildew. Methods. Molecular genetic (DNA isolation, polymerase chain reaction, electrophoresis of amplification products) and phytopathological methods. Results. A study of soft wheat varieties of Ukrainian breeding was carried out. Two csLV34a and csLV34b alleles were identified that correlate with the absence and presence of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene. 17.6 % of the cultivars tested were found to have the csLV34b allele, which is associated with the presence of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene and resistance to brown rust. Conclusions. PCR revealed six varieties of soft wheat that contain the allele csLV34b, which is associated with the presence of the gene Lr34/Yr18/Sr57/Pm38/Bdv1. The information obtained can be used in breeding programs to create new varieties resistant to disease.
Keywords: leaf rust, powdery mildew, soft wheat, polymerase chain reaction, varieties, stability.