Open AccessCreation of new forms of feeding cereal grasses from A to Z
Author(s) -
I. P. Kondratskaya,
V. A. Stolepchenko,
A. N. Yuknimuk,
P. P. Vasko,
O. V. Chizhik,
Z. H. Kozlovskaya,
A. M. Deeva,
T. V. Mazur,
В. Н. Решетников,
E. A. Voyzehovska,
H. A. Pryadkina,
S. Vasiljevic
Publication year - 2020
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v27.1310
Subject(s) - biology , agropyron cristatum , agropyron , cultivar , agronomy , microbiology and biotechnology
Aim. To create a new cultivars of perennial cereal grasses with the high feeding and seed productivity, longevity, drought resistance and freezing tolerance in order to increase the quality of livestock super-elite seeds production. DNA certification of economically valuable properties estimation. Methods. Hybridization, a partially known combination. Embryo culture method from immature caryopsis with completion of growing in a nutrient medium. Techniques of plant tissue culture (initiation, multiplication, rooting and adaptation in vitro plants to ex vitro conditions. DNA certification was performed using SSR (Simple Sequence Repeat) marker system. Results. The new competitively cultivar of Agropyron cristatum "Malarytsky" have been created. The system of the cultivar and parental forms registration in the form of molecular genetic passports is presented. Conclusions. The cultivar of Agropyron cristatum "Malarytsky" was turned over to the State Institution “The State Inspection for Testing and Protection of Plant Varieties” for state testing and for inclusion in the State Register of Varieties. The economic efficiency of this new feeding culture cultivating for Belarus and countries with sharply continental climate is presented.
Keywords: Agropyron cristatum, hybridization, cul populations, in vitro, water-soluble carbohydrates, DNA certification, molecular genetic passports, cultivar.