Open AccessPCR-identification of bacteria belonging to the genus Pectobacterium – agents of cucumber soft rot and wilting in Ukraine
Author(s) -
L. A. Dankevych
Publication year - 2020
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v27.1303
Subject(s) - pectobacterium carotovorum , wilting , pectobacterium , biology , microbiology and biotechnology , polymerase chain reaction , genome , strain (injury) , botany , gene , genetics , pathogen , anatomy
Aim. Correct species identification of isolated Pectobacterium sp., collection «E. toxica» strains and typical representatives of some species of the genus Pectobacterium and Dickeya via PCR for individual species-specific regions of their genome. Methods. Microbiological and molecular genetic (PCR) methods Results. A specific PCR product of size 434 bp was amplified in the genome of isolated Pectobacterium sp., collection «E. toxica» and typical P. carotovorum susp. carotovorum UKM B1075T and P. atrosepticum UKM B-1084T strains. The 690 bp DNA fragment was detected solely in the genome of the typical P. atrosepticum UKM B-1084T strain and absent in strains which are agents of cucumber soft rot and wilting and a typical P. carotovorum susp. carotovorum UKM B1075T strain. Conclusions. PCR detection of specific DNA fragments allowed us to finally clarify the species status of the causative agent of cucumber soft rot and wilting and attribute it to P. carotovorum.
Keywords: identification, causative agent of cucumber soft rot and wilting.