Open AccessOptimization of Agrobacterium-mediated transformation of perspective winter wheat genotypes in vitro
Author(s) -
О. В. Дубровна,
L. V. Slivka
Publication year - 2020
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v26.1264
Subject(s) - agrobacterium , callus , kanamycin , transformation (genetics) , biology , genotype , in vitro , horticulture , botany , microbiology and biotechnology , genetics , antibiotics , gene
Aim. Optimization of conditions for genetic transformation of new perspective winter wheat genotypes. Methods. Agrobacterium-mediated transformation in culture in vitro. Results. The influence of the optical density of cells of the agrobacterial suspension, the concentration of the antibiotic cefotaxime, the duration of coculture on the frequency of obtaining kanamycin-resistant regenerants of new winter wheat genotypes by genetic transformation of callus cultures were investigated using LBA4404 and AGL0 strains. It is shown that depending on the strain the most optimal is the concentration of agrobacteria 0.2-03 OD, duration of coculture for 2-3 days and the use of cefotaxime at a concentration of 250-500 mg/L. Conclusions. The optimal parameters for conducting Agrobacterium-mediated transformation of callus cultures of new perspective winter wheat genotypes were selected.
Keywords: Triticum aestivum, Agrobacterium-mediated transformation, callus cultures.