Open AccessMolecular characterization of high-molecular weight glutenin subunits 1Bx6.1 and 1By22.1 from Triticum spelta К1731 accession
Author(s) -
О. A. Orlovskaya,
K. K. Yatsevich,
С. И. Вакула,
Л. В. Хотылева,
A. V. Kilchevsky
Publication year - 2019
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v25.1156
Subject(s) - glutenin , biology , gene , gluten , locus (genetics) , sanger sequencing , gel electrophoresis , biochemistry , protein subunit , genetics , dna sequencing
Aim. Some spelt varieties, along with alleles of gliadins and high-molecular glutenin subunits (HMW-GS), identical to common wheat, contain specific alleles, that are source of Triticum aestivum gene pool enrichment. The aim of this work is the identification, molecular analysis of HMW – GS from T. spelta K1731 and evaluation of their effect on the elastic properties of gluten. Methods. Identification of HMW-GS was carried out by SDS-electrophoresis and PCR analysis. Nucleotide gene sequences were determined by Sanger sequencing. The secondary structure of proteins was predicted on the on-line CFSSP server. Results. Subunits 6.1 + 22.1 of the Glu-B1 locus encoded by the Glu-B1be allele were detected in the T. spelta K1731. The nucleotide sequences of the 1Bx6.1, 1By22.1 genes from spelt were determined, the amino acid sequence and the protein secondary structure of 6.1 + 22.1 subunits were analyzed. Conclusions. Molecular analysis of HMW-GS 1Bх6.1 and 1By22.1 from T. spelta К1731 established a low contribution to the bread-making quality of these subunits.
Keywords: Triticum spelta K1731, HMW-GS, SDS electrophoresis, sequencing, secondary protein structure, gluten quality.