Open AccessGenetic potential of bacteria isolated from cadmium contaminated soils
Author(s) -
Olga Iungin,
О. Yu. Bielikova,
G.V. Gladka,
Oleksandr Tashyrev
Publication year - 2018
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v23.1040
Subject(s) - cadmium , bacteria , biology , contamination , microbiology and biotechnology , food science , chemistry , genetics , ecology , organic chemistry
Aim. Determination of taxonomic position and resistance to Cd2+, Cu2+, Ni2+, Co2+, CrO42- of isolated bacteria from cadmium-contaminated soils. Methods. Bacterial cultures were isolated by ten-fold dilutions and further cultivation on agarized nutrient medium. Taxonomic position of isolated cultures was determined by molecular-genetic methods using GenBank and BLAST. Bacterial resistance to toxic metals was determined by strains cultivation in liquid medium in the presence of concentration gradient of Cd2+, Cu2+, Ni2+, Co2+, CrO42-. Results. Isolated cultures were highly resistance to cadmium. These bacterial cultures were identified as Brevundimonas vesicularis and Cupriavidus gilardii by phenotypic and molecular-genetic characteristics. Their resistance was in one order higher comparing to generally accepted. Conclusions. Isolated strains are promising for using in novel industrial biotechnologies for metal containing wastewater treatment.
Keywords: metalresistance, toxic metals, taxonomic position, Cupriavidus gilardii, Brevundimonas vesicularis.