The frequency of T-DNA integration during the genetic transformation of tobacco (Nicotiana tabacum L.)

Aim. Investigation of the is integration frequency of the genes and their components (elements of genetic constructions) in transgenic tobacco-regenerating plants. Methods. Agrobacterium-mediated transformation of tobacco (Nicotiana tabacum L.) in vitro. Results. There were obtained tobacco plants with integrated target and selective genes. It was found that a complete copy of T-DNA or its certain elements were embedded. Conclusions. The possibility of the integration of complete and incomplete DNA copies during in vitro Agrobacterium-mediated transformation of the Nicotiana tabacum were established. This event were observed both during manipulations with pBi2E plasmid with the double-stranded RNA PHD gene suppressor and pBi-OAT plasmid with OAT gene. The transformation frequency with the complete integration of the genetic construction was 91 %. The integration frequencies for various plasmids were: LBA4404 pBi2E – 78 %; AGLO pBi2E – 100 %; AGLO pBi-OAT – 94 %. Keywords: Nicotiana tabacum L., Agrobacterium-mediated transformation in vitro, transgenic plants, T-DNA.