Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene | Zendy

O. M. Honcharuk | Zendy; О. В. Дубровна | Zendy

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Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

Author(s) -

O. M. Honcharuk,

О. В. Дубровна

Publication year - 2018

Publication title -

faktori eksperimental noi evolucii organizmiv

Language(s) - English

Resource type - Journals

eISSN - 2415-3826

pISSN - 2219-3782

DOI - 10.7124/feeo.v22.952

Subject(s) - transformation (genetics) , agrobacterium , biology , ornithine aminotransferase , callus , genetically modified crops , gene , transgene , heterologous , expression vector , genome , botany , ornithine , genetics , recombinant dna , amino acid , arginine

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

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