Cardiac deletion of α-E-catenin leads to redused expression of the main component of desmosomes – γ-catenin | Zendy

Volodymyr V. Balatskyi | Zendy; L. L. Matsevych | Zendy; О. O. Piven | Zendy

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Cardiac deletion of α-E-catenin leads to redused expression of the main component of desmosomes – γ-catenin

Author(s) -

Volodymyr V. Balatskyi,

L. L. Matsevych,

О. O. Piven

Publication year - 1970

Publication title -

faktori eksperimental noi evolucii organizmiv

Language(s) - English

Resource type - Journals

eISSN - 2415-3826

pISSN - 2219-3782

DOI - 10.7124/feeo.v21.854

Subject(s) - catenin , genetically modified mouse , downregulation and upregulation , knockout mouse , conditional gene knockout , transgene , biology , messenger rna , gene expression , gene , beta catenin , western blot , microbiology and biotechnology , wnt signaling pathway , genetics , phenotype

Aim. In our present work, we have addressed to the γ-catenin, known main component of desmosomes, expression in hearts with heterozygous and homozygous knockout of α-E-catenin gene. Methods. Alpha-E-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. We analyze expression of γ-catenin with real time qPCR and Western blot. Results. Cardiac α-E-catenin deletion leads to downregulation of γ-catenin mRNA and protein levels only in homozygous mice, while we not observed any perturbation of γ-catenin expression in heterozygous mice. Conclusions. We have shown that homozygous knockout of α-E-catenin gene in embryonic heart occur reduction of the main component of desmosomes – γ-catenin mRNA and protein level of expression, which can lead to disruption of the desmosomes structure in adult myocardium. Keywords: α-E-catenin, heart failure, γ-catenin.

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