Open AccessCardiac deletion of α-E-catenin leads to redused expression of the main component of desmosomes – γ-catenin
Author(s) -
Volodymyr V. Balatskyi,
L. L. Matsevych,
O. O. Piven
Publication year - 1970
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v21.854
Subject(s) - catenin , genetically modified mouse , downregulation and upregulation , knockout mouse , conditional gene knockout , transgene , biology , messenger rna , gene expression , gene , beta catenin , western blot , microbiology and biotechnology , wnt signaling pathway , genetics , phenotype
Aim. In our present work, we have addressed to the γ-catenin, known main component of desmosomes, expression in hearts with heterozygous and homozygous knockout of α-E-catenin gene. Methods. Alpha-E-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. We analyze expression of γ-catenin with real time qPCR and Western blot. Results. Cardiac α-E-catenin deletion leads to downregulation of γ-catenin mRNA and protein levels only in homozygous mice, while we not observed any perturbation of γ-catenin expression in heterozygous mice. Conclusions. We have shown that homozygous knockout of α-E-catenin gene in embryonic heart occur reduction of the main component of desmosomes – γ-catenin mRNA and protein level of expression, which can lead to disruption of the desmosomes structure in adult myocardium.
Keywords: α-E-catenin, heart failure, γ-catenin.