Open AccessDetermination of allelic variants of gene Glu-1 in hybrid families, bearing gene Gpc-B1 of Triticum turgidum ssp. dicoccoides
Author(s) -
S. Yu. Pokhylko,
Anton Stepanenko,
О. М. Дуган,
B.V. Morgun
Publication year - 1970
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v21.829
Subject(s) - triticum turgidum , biology , genetics , allele , gene , polymerase chain reaction , gene pool , genetic diversity , population , medicine , environmental health
Aim. The aim of our study was to analyze the hybrid families of generation F5 carrying gene Gpc-B1 of Triticum turgidum ssp. dicoccoides for the allelic state of Glu-1, to calculate 1000 kernel weight and to select families with the best set of alleles and performance. Methods. Polymerase chain reaction for molecular genetic analysis and calculation of the 1,000-kernel weight for wheat grain yields were used. Results. Determination of the three loci Glu-A1, Glu-B1, Glu-D1 shows that among 44 hybrid families it is possible to identify 16 most promising families that have the most valuable allelic variants. Furthemore, given the analysis of the 1,000-kernel weight, two families (#36 and 40) having the highest values can be selected among those 16 families. Conclusions. The results of study enable a comprehensive approach to the selection of progeny with the best genetic base, which will be used in breeding of soft winter wheat later on.
Keywords: Triticum aestivum L., gene Gpc-B1, Glu-1 loci, PCR, molecular markers.