Open AccessChoice lines of Drosophila melanogaster with reporter system sensitive to low doses of mutagens
Author(s) -
Ya. S. Yasinskyi,
Л. В. Омельянчук,
О. В. Жук,
Iryna Kozeretska
Publication year - 1970
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v21.811
Subject(s) - reporter gene , drosophila melanogaster , ionizing radiation , mutagen , biology , microbiology and biotechnology , gene , genetics , carcinogen , irradiation , gene expression , physics , nuclear physics
Aim. There are many methods of assessment of mutagens, but the effects of low-dose mutagens have been overlooked. In this study we try to select lines of Drosophila melanogaster with high-sensitive to mutagen reporter gene. We used D. melanogaster lines with enhancer trap P {lacW} which produces beta-galactosidase gene in response to activation induced reparation. Methods. Ionizing radiation in dose of 100 roentgens were used as a mutagen. We extracted salivary glands of the third instar of the fruit fly larvae. The sample was fixed with 0.75 % glutaraldehyde and stained by 12-hour incubation with 0.2 % X-Gal at 37°C. Activity of the reporter gene was determined by microscope. Results. Two of the three lines are not shown according to the activation of the reporter gene from the resulting dose of ionizing radiation. In line y [1] w [67c23]; P {w [+ mC] = lacW} mus209 [k00704] / CyO (#10361) activation of the reporter gene occurred in some nuclei salivary gland after radiation. Conclusions. The reporter gene P{lacW} may be used for the detection of small doses of mutagens. Larvae of line #10361 has reporter gene activation in response to ionizing radiation.
Keywords: mutagenic factors, Drosophila melanogaster, LacZ reporter, radiation.