Open AccessLocomotor activity of Drosophila melanogaster larvae with altered function of gene swiss cheese
Author(s) -
N. P. Matiytsiv,
Olena Trush,
O. M. Gudyma,
Y. I. Chernyk
Publication year - 1970
Publication title -
faktori eksperimentalʹnoï evolûcìï organìzmìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3826
pISSN - 2219-3782
DOI - 10.7124/feeo.v21.803
Subject(s) - drosophila melanogaster , biology , knockout mouse , gene knockout , melanogaster , transgene , gene , neuroscience , genetics
Aim. Gene swiss cheese (sws) in Drosophila melanogaster is ortholog of human gene Neuropathy Target Esterase (NTE). Point mutation in this gene lead to many neurodegenerative disorders, such as Spastic Paraplegia. The aim of this study was to determine whether the change of gene sws function influence in Drosophila larvae motor behavior. Methods. We used two mutant lines: sws1 and sws76-15; transgenic lines for UAS-Gal4 controlled expression of SWS in moroneurons. Locomotor assay was performed in third-instar larvae. Results. We have found that larvae sws76-15 and sws1 showed significant decrease of motor behavior in comparison with wild type: sws1 has 35.4 %, and sws76-15 24.1 % difference with Oregon R. Functional knockout of sws in motoneuron showed decrease of motor activity as well – 16.5 % less than control. overexpression sequences of complete SWS protein in motoneurons causes significant increment in motor activity about 43.7 %. Esterase domen and three nucleotidbinding domains overexpression in larvae motoneurons had no differences compared to control. Conclusions. For the first time was shown a critical role of gene sws for larvae motor activity in D. melanogaster. Flies with altered sws function are good model of spastic paraplegia study.
Keywords: Drosophila melanogaster, sws/NTE, neuropathy, locomotor behavior.