Open AccessTritium-labeled compounds II. General-purpose apparatus, and procedures for the preparation, analysis, and use of tritium oxide and tritium-labeled lithium borohydride
Author(s) -
Horace S. Isbell,
J. D. Moyer
Publication year - 1959
Publication title -
journal of research of the national bureau of standards. section a. physics and chemistry
Language(s) - English
Resource type - Journals
eISSN - 2376-5704
pISSN - 0022-4332
DOI - 10.6028/jres.063a.012
Subject(s) - tritium , chemistry , radiochemistry , lithium (medication) , tritiated water , borohydride , tritium illumination , nuclear chemistry , organic chemistry , nuclear physics , catalysis , physics , endocrinology , medicine
A general-purpose manifold is described, which is useful for numerous procedures involving tritium gas and tritium-labeled materials. Methods are given in detail for (a) converting tritium gas to tritium oxide, (b) preparing tritium-labeled lithium borohydride, and (c) conducting a variety of reactions in a closed system. Auxiliary equipment is shown, including water traps, reaction flasks, and apparatus for preparing solutions and making filtrations in closed systems. Methods are presented for assaying tritium-labeled water by (a) dissolving it in a phosphoric anhydride-sulfuric acid solution and counting in a windowless, gas-flow, proportional counter, or (b) converting it to hydrogen- t and assaying it in an ionization chamber. The use of tritium-labeled lithium borohydride is illustrated by the preparation of d-galactitol- 1 - t from d-galactose. Subsequent papers will describe the use of this apparatus in the synthesis of tritium-labeled carbohydrates. The detailed description of techniques and apparatus should be helpful to others who are interested in using tritium.