Open AccessAntimicrobial resistance genes and bacterial diversity in sediments of Guandu River, Brazil
Author(s) -
Gustavo Souza Lima Sant’Anna,
José Maurício Fajardo da Cunha,
Juliana Ferreira Nunes,
Isabel Arjonas Fernandes Avila,
João Vitor da Silva Gonçalves,
Paula Fernanda Alves Ferreira,
Miliane Moreira Soares de Souza,
Shana de Mattos de Oliveira Coelho,
Irene da Silva Coelho
Publication year - 2021
Publication title -
revista ibero-americana de ciências ambientais
Language(s) - English
Resource type - Journals
ISSN - 2179-6858
DOI - 10.6008/cbpc2179-6858.2021.004.0012
Subject(s) - antimicrobial , biology , antibiotic resistance , tetracycline , proteobacteria , gene , microbiology and biotechnology , ecology , antibiotics , genetics , 16s ribosomal rna
Rivers provide essential water resources for humans. However, appear to be reservoirs of antimicrobial-resistant genes and dispersal routes of resistant pathogens because human activities promptly influence them. Sediments are the most propitious substrates for bacteria survival and can act as an antimicrobial reservoir. Three sediment samples were collected upstream of the water catchment point of the Guandu Water Treatment Plant (ETA Guandu) to evaluate microbial diversity and antimicrobial resistance genes to sulfonamide (sul1, sul2), tetracycline (tetA, tetB) and beta-lactam (blaCTX-M, blaoxa24 and blaoxa58) by PCR. The most abundant phylum in all samples was Proteobacteria, which was also the most dominant in sediments presenting a pattern already reported in the literature for diversity in tropical and temperate climate rivers. Sulfonamide resistance genes were detected in all samples. The blaCTX-M gene was detected only in one sample, and tetA, tetB, blaoxa24 and blaoxa58 genes were not found in any sample. The presence of antimicrobial resistance genes sul1, sul2, and blaCTX-M indicate that sediments can act as a reservoir of resistance. These findings will provide new data about the bacterial community and antimicrobial resistance genes in Brazilians river sediments.