Open AccessAntifungal activity of paracloacal gland secretion of Caiman yacare (DAUDIN, 1802)
Author(s) -
Lucas Polizzeli Azevedo,
Leandro Nogueira Pressinotti,
Rhavena Graziela Liotti,
Célia Alves de Souza,
Elaine Maria Loureiro,
Carina Aparecida Pinto,
Natasha Rayane de Oliveira Lima,
Mariana Lenina Menezes Aleixo
Publication year - 2018
Publication title -
revista ibero-americana de ciências ambientais
Language(s) - English
Resource type - Journals
ISSN - 2179-6858
DOI - 10.6008/cbpc2179-6858.2018.001.0010
Subject(s) - antifungal , minimum inhibitory concentration , candida albicans , biology , dilution , aqueous extract , aqueous solution , resazurin , chromatography , corpus albicans , microbiology and biotechnology , chemistry , in vitro , traditional medicine , biochemistry , medicine , organic chemistry , physics , thermodynamics
The objective of this study was to evaluate the in vitro antifungal activity of ethanolic and aqueous extracts obtained from Caiman yacare paracloacal gland (PG) against Candida albicans (ATCC 10231), as well as a bibliographical survey on the chemical composition of exudates of PG. The PG were collected from the disposal generated during the slaughter of C. yacare by regularized industry. Two extracts were made from these glands, one ethanolic and the other aqueous. The Minimal Inhibitory Concentration (MIC) of the substances were determined by dilution of the extract in series using the microdilution technique in the culture medium Sabouraud broth, carried out in a 96-well microplate visually read after 48 hours of incubation, confirmed by the method using 0.01% aqueous resazurin dye. The ethanolic extract had MIC at the concentration of 25 µg / L. The Minimum Fungicidal Concentration (MFC) was determined by subcultures of MIC in Sabouraud agar medium. The ethanolic extract presented MFC at a concentration of 50 µg / mL. The aqueous extract showed no antifungal activity at the concentrations tested. This work is the first work to assess an activity of the PG secretion and reveals pharmacological potential in a local product previously discarded.