Open AccessConcentration effect of polyethylene glycol in evaluation of grain legumes for drought tolerance
Author(s) -
Nadiia Vus,
Antonina Vasylenko,
Vyacheslav Lutenko,
Л. Н. Кобызева,
Olha Bezuhla,
Larisa Shevchenko,
С. Г. Понуренко,
Feng Baili,
Dmitry Saliy
Publication year - 2020
Publication title -
žemės ūkio mokslai
Language(s) - English
Resource type - Journals
eISSN - 2424-4120
pISSN - 1392-0200
DOI - 10.6001/zemesukiomokslai.v27i2.4337
Subject(s) - peg ratio , germination , polyethylene glycol , agronomy , drought tolerance , crop , biology , horticulture , biochemistry , finance , economics
The article covers selection of differentiating concentrations of PEG-6000 for assessing genetic resource collections of pea, chickpea and lentil. The germinability of 4 accessions of each crop in 5, 10, 15, 20 and 25% PEG-6000 solutions was evaluated. The results showed that 25% PEG-6000 completely inhibited growth processes in all the crops; 5 and 10% PEG-6000 did not affect the germinability of lentil seeds; and the maximum differentiation was observed at an osmotic concentration of 20%. In chickpea, there were no seedlings even in 20% PEG-6000. In 15% PEG-6000, seeds of drought-tolerant accessions UD0500022 and Dnіprovskyi Vysokoroslyi only sprouted; and 5 and 10% solutions had the maximum differentiating effect. Pea germination in PEG-6000 solutions of different concentrations demonstrated that only one accession could germinate in 20 and 15% solutions. Two accessions gave seedlings in 10% PEG-6000, and 5% solution had almost no effect on the germinability of pea seeds.