Open AccessBiotechnological esterification of oleic acid with 2-ethylhexanol in biolubricant synthesis technologies
Author(s) -
Ieva Čepaitė,
Milda Gumbytė
Publication year - 2017
Publication title -
žemės ūkio mokslai
Language(s) - English
Resource type - Journals
eISSN - 2424-4120
pISSN - 1392-0200
DOI - 10.6001/zemesukiomokslai.v24i1.3442
Subject(s) - oleic acid , lipase , chemistry , grease , organic chemistry , chromatography , molar ratio , enzyme , catalysis , biochemistry
The possibilities of waste materials re-use in biosynthesis of grease were investigated. In this paper, 2-ethylhexanololeate synthesis from 2-ethylhexanol and oleic acid using industrial enzymes is discussed. In order to select an effective enzyme for the mentioned synthesis, the selection of industrial lipases was carried out. During the reactions, acidity of the substances was measured and a chromatographic analysis was held to observe the reaction rate. After choosing the suitable lipase (Lipozyme TL IM), an experimental model was created. When all reactions from the plan were completed, optimal conditions were estimated. The process temperature was 41.04 °C, the duration of the process was 26.10 h, the molar ratio of 2-ethylhexanololeate and oleic acid was 2.35:1.16 mol/mol, and the enzyme quantity (according to acid quantity) was 9.91%.