Open AccessCirsium vulgare leaves: isolation and identification of phenolic compounds
Author(s) -
Urtė Griškevičienė,
Mindaugas Marksa,
Augusta Ževžikovienė,
Daiva Kazlauskienė,
Rimanta Vainorienė,
Andrejus Ževžikovas,
Liudas Ivanauskas
Publication year - 2021
Publication title -
chemija
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 15
eISSN - 2424-4538
pISSN - 0235-7216
DOI - 10.6001/chemija.v32i3-4.4547
Subject(s) - nutraceutical , maceration (sewage) , extraction (chemistry) , chromatography , chemistry , materials science , food science , composite material
There are a few new researches on the pharmaceutical and nutraceutical application of Cirsium vulgare, but they do not reflect the full potential use of this plant. For the best of our knowledge, no study has been conducted so far to determine the impact of extraction methods and conditions on the extraction yields of phenolic compounds from Cirsium vulgare. We aimed to investigate a simple and sensitive HPLC-PDA method to determine phenolic compounds in Cirsium vulgare extracts. Three extraction techniques as maceration (ME), ultrasound-assisted extraction (UAE) and heat-reflux extraction (HRE) were applied and compared for sample preparation to obtain a high recovery of the phenolic compounds. The developed HPLC-PDA method can be used for the preparations of extracts with a high content of bioactive compounds for both pharmaceutical and nutraceutical applications. Moreover, it was validated and used for the qualitative and quantitative evaluation of phenolic compounds. A simple extraction procedure for phenolic compounds from Cirsium vulgare leaves was optimized in this work through the use of the heatreflux extraction method with 50% ethanol and extraction time of 1.5 h.