Open AccessBiodistribution and Clearance of Human Mesenchymal Stem Cells by Quantitative Three‐Dimensional Cryo‐Imaging After Intravenous Infusion in a Rat Lung Injury Model
Author(s) -
Schmuck Eric G.,
Koch Jill M.,
Centanni John M.,
Hacker Timothy A.,
Braun Rudolf K.,
Eldridge Marlowe,
Hei Derek J.,
Hematti Peiman,
Raval Amish N.
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0379
Subject(s) - biodistribution , mesenchymal stem cell , spleen , medicine , lung , pathology , stem cell , cell , nuclear medicine , in vivo , chemistry , biology , genetics , microbiology and biotechnology , biochemistry
To study three‐dimensional (3D) cryo‐imaging to measure cell biodistribution and clearance after intravenous infusion, the authors established a lung injury model in rats. Human mesenchymal stem cells (hMSCs) labeled with QTracker were infused via jugular vein. Organs were cryopreserved, followed by 3D cryo‐imaging. At 60 minutes, 82 ± 9.7% of cells were detected, and at day 2, 0.06% of cells were detected. hMSCs were retained primarily in the liver, with fewer detected in lungs and spleen.