Open AccessV‐Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A Synthetic Modified mRNA Drives Reprogramming of Human Pancreatic Duct‐Derived Cells Into Insulin‐Secreting Cells
Author(s) -
Corritore Elisa,
Lee Yong-Syu,
Pasquale Valentina,
Liberati Daniela,
Hsu Mei-Ju,
Lombard Catherine Anne,
Van Der Smissen Patrick,
Vetere Amedeo,
Bonner-Weir Susan,
Piemonti Lorenzo,
Sokal Etienne,
Lysy Philippe A.
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0318
Subject(s) - reprogramming , pancreatic duct , fibrosarcoma , ht1080 , oncogene , microbiology and biotechnology , biology , cancer research , gene , endocrinology , pancreas , cell cycle , genetics
This study shows a novel and safe method for inducing β‐cell differentiation from expanded human pancreatic duct‐derived cells by using MAFA synthetic modified mRNA‐based reprogramming. The resulting cells showed glucose‐dependent insulin secretion both in vitro and after transplantation into diabetic animals, where they led to significant and prompt reduction of blood glucose levels.