Open AccessHuman Induced Pluripotent Stem Cells Differentiate Into Functional Mesenchymal Stem Cells and Repair Bone Defects
Author(s) -
Sheyn Dmitriy,
Ben-David Shiran,
Shapiro Galina,
De Mel Sandra,
Bez Maxim,
Ornelas Loren,
Sahabian Anais,
Sareen Dhruv,
Da Xiaoyu,
Pelled Gadi,
Tawackoli Wafa,
Liu Zhenqiu,
Gazit Dan,
Gazit Zulma
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0311
Subject(s) - induced pluripotent stem cell , mesenchymal stem cell , microbiology and biotechnology , stem cell , reprogramming , embryoid body , clinical uses of mesenchymal stem cells , osteoblast , stem cell transplantation for articular cartilage repair , cellular differentiation , bone marrow , adult stem cell , biology , immunology , embryonic stem cell , cell , in vitro , genetics , gene
Using short‐term exposure of embryoid bodies to transforming growth factor‐β, the authors directed induced pluripotent stem cells (iPSCs) toward mesenchymal stem cell (MSC) differentiation. Two types of iPSC‐derived MSCs were identified: early (aiMSCs) and late (tiMSCs) outgrowing cells. Both types differentiated in vitro in response to osteogenic or adipogenic supplements; aiMSCs demonstrated higher osteogenic potential than tiMSCs. Upon orthotopic injection into radial defects, both types regenerated bone and contributed to defect repair.