Open AccessRho Kinase Inhibition Is Essential During In Vitro Neurogenesis and Promotes Phenotypic Rescue of Human Induced Pluripotent Stem Cell‐Derived Neurons With Oligophrenin‐1 Loss of Function
Author(s) -
Compagnucci Claudia,
Barresi Sabina,
Petrini Stefania,
Billuart Pierre,
Piccini Giorgia,
Chiurazzi Pietro,
Alfieri Paolo,
Bertini Enrico,
Zanni Ginevra
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0303
Subject(s) - neurogenesis , induced pluripotent stem cell , microbiology and biotechnology , phenotype , stem cell , biology , function (biology) , in vitro , kinase , gene , genetics , embryonic stem cell
This study showed the morphological, biochemical, and functional features of human OPHN1‐deficient induced pluripotent stem cells characterized by hyperactive rho kinase (ROCK) signaling and their rescue by treatment with the ROCK inhibitor fasudil. These findings shed light on the relevance of the ROCK pathway during in vitro neuronal differentiation in physiology and disease, not only through morphological changes due to cytoskeletal reorganization but also through epigenetic regulation that allows transcription of genes that are relevant for neuronal differentiation and survival, such as NR4A1 .