Open AccessModeling Cystic Fibrosis Using Pluripotent Stem Cell‐Derived Human Pancreatic Ductal Epithelial Cells
Author(s) -
Simsek Senem,
Zhou Ting,
Robinson Christopher L.,
Tsai Su-Yi,
Crespo Miguel,
Amin Sadaf,
Lin Xiangyi,
Hon Jane,
Evans Todd,
Chen Shuibing
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0276
Subject(s) - induced pluripotent stem cell , cystic fibrosis transmembrane conductance regulator , embryonic stem cell , stem cell , cystic fibrosis , biology , microbiology and biotechnology , chloride channel , stem cell therapy , cellular differentiation , cancer research , pathology , medicine , genetics , gene
This report describes a method of directing human pluripotent stem cells, including human embryonic stem cells (hESCs) and an induced pluripotent stem cell line derived from patients with cystic fibrosis, to differentiate into pancreatic ductal epithelial cells (PDECs). After purification, more than 98% of hESC‐derived PDECs expressed functional cystic fibrosis transmembrane conductance regulator protein. This method may help identify drug candidates to rescue the pancreatic defects of patients with cystic fibrosis.