Open AccessRapamycin‐Induced Hypoxia Inducible Factor 2A Is Essential for Chondrogenic Differentiation of Amniotic Fluid Stem Cells
Author(s) -
Preitschopf Andrea,
Schörghofer David,
Kinslechner Katharina,
Schütz Birgit,
Zwickl Hannes,
Rosner Margit,
Joó József Gabor,
Nehrer Stefan,
Hengstschläger Markus,
Mikula Mario
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0262
Subject(s) - chondrogenesis , microbiology and biotechnology , stem cell , hypoxia inducible factors , amniotic fluid , hypoxia (environmental) , stem cell factor , biology , cellular differentiation , chemistry , fetus , pregnancy , haematopoiesis , genetics , gene , organic chemistry , oxygen
This study investigated the effects of mammalian target of rapamycin complex 1 (mTORC1) modulation on the expression of SOX9 and its downstream targets during chondrogenic differentiation of amniotic fluid stem (AFS) cells. mTORC1 inhibition led to upregulation of chondrogenic markers during AFS cell differentiation and during redifferentiation of in vitro‐expanded human chondrocytes.