Open AccessFunctional Maturation of Induced Pluripotent Stem Cell Hepatocytes in Extracellular Matrix—A Comparative Analysis of Bioartificial Liver Microenvironments
Author(s) -
Wang Bo,
Jakus Adam E.,
Baptista Pedro M.,
Soker Shay,
Soto-Gutierrez Alejandro,
Abecassis Michael M.,
Shah Ramille N.,
Wertheim Jason A.
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0235
Subject(s) - bioartificial liver device , induced pluripotent stem cell , extracellular matrix , microbiology and biotechnology , stem cell , biology , chemistry , hepatocyte , embryonic stem cell , biochemistry , in vitro , gene
The ability of two three‐dimensional bioscaffold systems to reverse the primary limitations of induced pluripotent stem cell (iPSC)‐derived hepatocytes was compared. Proliferation and function of iPSC hepatocytes were significantly enhanced when cultured within scaffolds made from extracellular matrix (ECM). This ECM scaffold enhanced phenotypic maturation of iPSC hepatocytes compared with other platforms, likely owing to its biologically diverse makeup.