Open AccessStepwise Differentiation of Mesenchymal Stem Cells Augments Tendon‐Like Tissue Formation and Defect Repair In Vivo
Author(s) -
Yin Zi,
Guo Jia,
Wu Tian-yi,
Chen Xiao,
Xu Liang-liang,
Lin Si-en,
Sun Yun-xin,
Chan Kai-Ming,
Ouyang Hongwei,
Li Gang
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0215
Subject(s) - mesenchymal stem cell , ctgf , tendon , connective tissue , stem cell , microbiology and biotechnology , extracellular matrix , biology , transforming growth factor , cellular differentiation , growth factor , immunology , pathology , anatomy , medicine , gene , genetics , receptor
To induce tenogenesis of bone marrow‐derived mesenchymal stem cells (BMSCs), a stepwise tenogenic differentiation approach was established by first using transforming growth factor‐β1 stimulation, followed by combination with connective tissue growth factor. Gene expression analysis showed that this protocol initiated and maintained highly efficient tenogenesis of BMSCs. Tendons treated with induced tenogenic BMSCs had better structural and mechanical properties than controls.