Open AccessCancer Stem Cell‐Based Models of Colorectal Cancer Reveal Molecular Determinants of Therapy Resistance
Author(s) -
De Angelis Maria Laura,
Zeuner Ann,
Policicchio Eleonora,
Russo Giorgio,
Bruselles Alessandro,
Signore Michele,
Vitale Sara,
De Luca Gabriele,
Pilozzi Emanuela,
Boe Alessandra,
Stassi Giorgio,
Ricci-Vitiani Lucia,
Amoreo Carla Azzurra,
Pagliuca Alfredo,
Francescangeli Federica,
Tartaglia Marco,
De Maria Ruggero,
Baiocchi Marta
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0214
Subject(s) - cancer stem cell , colorectal cancer , cancer research , targeted therapy , cancer , in vivo , stem cell , biology , medicine , genetics
Colorectal cancer stem cell (CSC)‐enriched cultures were obtained efficiently from primary tumor specimens by optimizing a CSC‐isolation protocol. Based on in vitro and in vivo validation, genetic characterization, and drug sensitivity analysis, panels of CSC lines were generated with defined patterns of genetic mutations and therapy sensitivity. An analysis of CSC response to EGFR‐targeted therapy in vivo and an overview of factors implicated in therapy response/resistance are presented.