Open AccessMassive Clonal Selection and Transiently Contributing Clones During Expansion of Mesenchymal Stem Cell Cultures Revealed by Lentiviral RGB‐Barcode Technology
Author(s) -
Selich Anton,
Daudert Jannik,
Hass Ralf,
Philipp Friederike,
von Kaisenberg Constantin,
Paul Gabi,
Cornils Kerstin,
Fehse Boris,
Rittinghausen Susanne,
Schambach Axel,
Rothe Michael
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0176
Subject(s) - mesenchymal stem cell , biology , stem cell , flow cytometry , in vitro , clonal selection , microbiology and biotechnology , immunology , genetics
Mesenchymal stem cells (MSCs) have to be expanded in vitro for clinical use, which often leads to highly variable clinical benefit and experimental results. Multicolor fluorescence labeling and deep sequencing were used to demonstrate the dynamic clonal composition of MSC cultures, which might ultimately explain the variable clinical performance of the cells.