Open AccessControlled Dual Growth Factor Delivery From Microparticles Incorporated Within Human Bone Marrow‐Derived Mesenchymal Stem Cell Aggregates for Enhanced Bone Tissue Engineering via Endochondral Ossification
Author(s) -
Dang Phuong N.,
Dwivedi Neha,
Phillips Lauren M.,
Yu Xiaohua,
Herberg Samuel,
Bowerman Caitlin,
Solorio Loran D.,
Murphy William L.,
Alsberg Eben
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0115
Subject(s) - endochondral ossification , chondrogenesis , mesenchymal stem cell , chemistry , microbiology and biotechnology , cartilage , osteocalcin , tissue engineering , stem cell , growth factor , biomedical engineering , alkaline phosphatase , anatomy , biology , biochemistry , medicine , receptor , enzyme
A microparticle‐based growth factor delivery system was engineered to drive endochondral ossification within human bone marrow‐derived mesenchymal stem cell (hMSC) aggregates. Compared with cell‐only aggregates treated with exogenous growth factors, aggregates with incorporated transforming growth factor‐β1‐ and BMP‐2‐loaded microparticles exhibited enhanced chondrogenesis and alkaline phosphatase activity and a greater degree of mineralization. This microparticle‐incorporated system has potential as a readily implantable therapy for healing bone defects without the need for long‐term in vitro chondrogenic priming.