Open AccessDownregulation of Melanoma Cell Adhesion Molecule (MCAM/CD146) Accelerates Cellular Senescence in Human Umbilical Cord Blood‐Derived Mesenchymal Stem Cells
Author(s) -
Jin Hye Jin,
Kwon Ji Hye,
Kim Miyeon,
Bae Yun Kyung,
Choi Soo Jin,
Oh Wonil,
Yang Yoon Sun,
Jeon Hong Bae
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0109
Subject(s) - cd146 , mesenchymal stem cell , senescence , cell sorting , downregulation and upregulation , microbiology and biotechnology , stem cell , cancer research , alcam , biology , cell , cell adhesion molecule , immunology , flow cytometry , cd34 , biochemistry , genetics , gene
CD146 expression is gradually decreased during the long‐term expansion of human umbilical cord blood‐derived mesenchymal stem cells (hUCB‐MSCs) in culture, and enforced CD146 downregulation accelerated senescence in hUCB‐MSCs, which affected their multilineage differentiation potential, growth, and stemness. These data indicate a possible role for CD146 in inhibiting senescence in hUCB‐MSCs, which may occur via the regulation of Bmi‐1, Id1, and Twist1 expression. CD146 may be a novel marker for predicting senescence in hUCB‐MSCs, and it could be valuable in quality‐control assessments and for improving the therapeutic potential of hUCB‐MSC‐based therapy.