Open AccessStem Cell‐Derived Immature Human Dorsal Root Ganglia Neurons to Identify Peripheral Neurotoxicants
Author(s) -
Hoelting Lisa,
Klima Stefanie,
Karreman Christiaan,
Grinberg Marianna,
Meisig Johannes,
Henry Margit,
Rotshteyn Tamara,
Rahnenführer Jörg,
Blüthgen Nils,
Sachinidis Agapios,
Waldmann Tanja,
Leist Marcel
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0108
Subject(s) - induced pluripotent stem cell , neurotoxicity , neurite , high content screening , biology , stem cell , cell type , cell , in vitro toxicology , neuroscience , microbiology and biotechnology , computational biology , bioinformatics , toxicity , in vitro , medicine , embryonic stem cell , genetics , gene
A two‐step protocol to generate large lots of identical peripheral human neuronal precursors was characterized and adapted to the measurement of peripheral neurotoxicity. High content imaging allowed an unbiased assessment of cell morphology and viability. The peripheral neurotoxicity test established shows the potential of human stem cells for clinically relevant safety testing of drugs in use and of new emerging candidates.